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March 1, 1996Journal of Clinical MicrobiologyOpen Access

Immunodetection of Pneumocystis carinii in bronchoalveolar lavage specimens compared with methenamine silver stain

Irmeli Lautenschlager O. Lyytikäinen Liisa Jokipii A. M. M. Jokipii A. G. Maiche Tapani Ruutu P Tukiainen Petri Ruutu

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Abstract

Immunodetection of Pneumocystis carinii, based on immunofluorescence and use of a monoclonal antibody specific for an antigen located within the cyst wall and detectable after trypsin digestion only, was compared with a methenamine silver stain in 553 bronchoalveolar lavage specimens from immunosuppressed patients. P. carinii was found by immunofluorescence in 72 (86%) and by silver stain in 68 (81%) of the total of 84 positive samples detected by either or both of these methods.

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Lautenschlager et al. (1996) studied this question.

synapsesocial.com/papers/6a6f84b635aa2c282ce0e07a https://doi.org/10.1128/jcm.34.3.728-730.1996

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