Identifies a 180-kDa IQGAP-like protein involved in actin assembly with Cdc42 in sea urchin eggs.
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Extends IQGAP-like Cdc42 signaling to invertebrate eggs; hypothesis-generating for cytoskeletal roles in mammalian cardiovascular cells.
We isolated a gene homologous to human cdc42 (ucdc42) from a sea urchin cDNA library. The GTPgammaS-bound UCdc42 induced actin assembly in sea urchin egg extract. Proteins that are involved in this actin assembly system were searched using UCdc42-bound agarose beads. A 180-kDa protein (p180), which showed a homology to human IQGAPs, bound to the GTPgammaS-UCdc42 beads. Immunodepletion of p180 from the sea urchin egg extract abolished this actin assembly on the UCdc42 beads. Immunofluorescent localization of p180 was similar to that of the actin cytoskeleton in the egg cortex and it was concentrated in the cleavage furrow during cytokinesis. A possible role of p180 in actin assembly is discussed.
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Nishimura et al. (2003) studied this question.
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