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September 14, 2009Cell Motility and the Cytoskeleton

Hyperpolarization of the plasma membrane potential provokes reorganization of the actin cytoskeleton and increases the stability of adherens junctions in bovine corneal endothelial cells in culture

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Authors

VNVerónica NinJHJulio A. HernándezSCSilvia Chifflet

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Overview

Experimental study demonstrates that membrane hyperpolarization strengthens adherens junctions and compacts actin in corneal endothelial cells, indicating nonexcitable cell adaptation via voltage.

Key Points

  • To determine the effects of plasma membrane potential hyperpolarization on actin cytoskeletal remodeling and adherens junction stability in bovine corneal endothelial cells.
  • Induced membrane hyperpolarization in cultured bovine corneal endothelial cells and intact bovine corneas by substituting extracellular sodium with nondiffusible cations or adding valinomycin.
  • Quantified peripheral actin filament organization and compactness using fluorescence microscopy image analysis.
  • Challenged adherens junction stability using experimental calcium deprivation and temperature reduction.
  • Plasma membrane hyperpolarization prompted compaction of adherens junction-associated actin filaments toward the cell periphery.
  • Hyperpolarized endothelial cells displayed heightened adherens junction stability against calcium depletion and cold temperature challenges.
  • Parallel actin cytoskeletal reorganizations were confirmed in the endothelial cell layer of whole bovine corneas.

Cite This Study

Nin et al. (2009) studied this question.

synapsesocial.com/papers/6a87ddc79842aaac2ffb1558https://doi.org/10.1002/cm.20416
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