Membrane currents and the resting membrane potential in cultured bovine pulmonary artery endothelial cells.
Population
Cultured endothelial cells from calf pulmonary artery (CPAE cells), n=104
Design
Preclinical
Authors
Discussion
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Member takes
Implication
No clinical implications; leaves open relevance of these conductances to human pulmonary endothelium in vivo.
Structured PICO
P
Population
Cultured endothelial cells from calf pulmonary artery (CPAE cells), n=104
I
Intervention
Whole-cell patch-clamp technique with application of ion channel blockers (Ba2+, quinine, quinidine, TEA, NPPB, Gd3+) and hypertonic cell shrinkage
O
Outcome
Ionic conductances determining the resting membrane potentialsurrogate
The resting membrane potential of cultured bovine pulmonary artery endothelial cells is highly variable and determined by the balance of an inwardly rectifying K+ current, an outwardly rectifying Cl- current, and a non-selective background current.
Cite This Study
Voets et al. (1996) studied this question.