What does this research mean for the field?

Voltage-gated K+ (KV) channels, rather than Ca2+-activated (KCa) or ATP-sensitive (KATP) K+ channels, are primarily responsible for controlling resting membrane potential and intracellular calcium concentration in pulmonary arterial smooth muscle cells. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

August 1, 1995Circulation Research

Voltage-Gated K + Currents Regulate Resting Membrane Potential and Ca 2+ i in Pulmonary Arterial Myocytes

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Population

Rat pulmonary arterial smooth muscle cells (PASMCs)

Comparison

Pharmacological blockade of K+ channels using… vs Baseline/unblocked conditions

Design

Preclinical

Authors

XYXiao-Jian YuanLishui Maternity and Child Health Care Hospital

Discussion

Member takes

Implication

KV channels primarily regulate Em and [Ca2+]i in rat PASMCs; leaves open their role in human pulmonary vascular disease.

Key Points

To determine which potassium channel subtypes regulate resting membrane potential and intracellular calcium concentration in rat pulmonary arterial smooth muscle cells.
Performed patch-clamp electrophysiology and quantitative fluorescent microscopy on isolated rat pulmonary arterial smooth muscle cells.
Evaluated channel inhibitors, including 4-aminopyridine (4-AP, 5–10 mmol/L) for KV, charybdotoxin (ChTX, 10–20 nmol/L) for KCa, and glibenclamide (2–10 µmol/L) for KATP under varying extracellular and intracellular calcium concentrations.
Inhibition of KV channels by 4-aminopyridine reduced KV current, caused membrane depolarization, triggered calcium-dependent action potentials, and increased resting intracellular calcium concentration.
Neither charybdotoxin nor glibenclamide altered resting membrane potential or baseline intracellular calcium, though charybdotoxin pretreatment enhanced 4-AP-induced calcium increases.

Structured PICO

Population

Rat pulmonary arterial smooth muscle cells (PASMCs)

Intervention

Pharmacological blockade of K+ channels using 4-Aminopyridine (4-AP), charybdotoxin (ChTX), and glibenclamide

Comparator

Baseline/unblocked conditions

Outcome

Resting membrane potential (Em) and intracellular Ca2+ concentration ([Ca2+]i)surrogate

Voltage-gated K+ channels are the primary regulators of resting membrane potential and intracellular calcium in rat pulmonary arterial smooth muscle cells.

Cite This Study

Xiao-Jian Yuan (1995) studied this question.

synapsesocial.com/papers/6a195beec05413006f5838cd https://doi.org/10.1161/01.res.77.2.370

View Full Paper

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August 1, 1995Circulation Research

Voltage-Gated K + Currents Regulate Resting Membrane Potential and Ca 2+ i in Pulmonary Arterial Myocytes

View Full Paper

Ask AI

Bookmark

Population

Rat pulmonary arterial smooth muscle cells (PASMCs)

Comparison

Pharmacological blockade of K+ channels using… vs Baseline/unblocked conditions

Design

Preclinical

Authors

XYXiao-Jian YuanLishui Maternity and Child Health Care Hospital

Discussion

Member takes

Implication

KV channels primarily regulate Em and [Ca2+]i in rat PASMCs; leaves open their role in human pulmonary vascular disease.

Key Points

To determine which potassium channel subtypes regulate resting membrane potential and intracellular calcium concentration in rat pulmonary arterial smooth muscle cells.
Performed patch-clamp electrophysiology and quantitative fluorescent microscopy on isolated rat pulmonary arterial smooth muscle cells.
Evaluated channel inhibitors, including 4-aminopyridine (4-AP, 5–10 mmol/L) for KV, charybdotoxin (ChTX, 10–20 nmol/L) for KCa, and glibenclamide (2–10 µmol/L) for KATP under varying extracellular and intracellular calcium concentrations.
Inhibition of KV channels by 4-aminopyridine reduced KV current, caused membrane depolarization, triggered calcium-dependent action potentials, and increased resting intracellular calcium concentration.
Neither charybdotoxin nor glibenclamide altered resting membrane potential or baseline intracellular calcium, though charybdotoxin pretreatment enhanced 4-AP-induced calcium increases.

Structured PICO

Population

Rat pulmonary arterial smooth muscle cells (PASMCs)

Intervention

Pharmacological blockade of K+ channels using 4-Aminopyridine (4-AP), charybdotoxin (ChTX), and glibenclamide

Comparator

Baseline/unblocked conditions

Outcome

Resting membrane potential (Em) and intracellular Ca2+ concentration ([Ca2+]i)surrogate

Voltage-gated K+ channels are the primary regulators of resting membrane potential and intracellular calcium in rat pulmonary arterial smooth muscle cells.

Cite This Study

Xiao-Jian Yuan (1995) studied this question.

synapsesocial.com/papers/6a195beec05413006f5838cd https://doi.org/10.1161/01.res.77.2.370

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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