What question did this study set out to answer?

To examine how sialylation alters the molecular structure and epitope expression of Neisseria gonorrhoeae lipooligosaccharide in vitro and during cellular interactions.

May 1, 1990The Journal of Experimental MedicineOpen Access

In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.

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Authors

RMRobert E. MandrellAgricultural Research Service ALAlan J. LesseVA Western New York Healthcare System JSJames V. SugaiUniversity of Michigan

Discussion

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Implication

Laboratory study demonstrates sialylation alters Neisseria gonorrhoeae lipooligosaccharide surface epitopes, indicating an endogenous mechanism for immune evasion during infection.

Key Points

To examine how sialylation alters the molecular structure and epitope expression of Neisseria gonorrhoeae lipooligosaccharide in vitro and during cellular interactions.
Cultured gonococci in the presence or absence of cytidine monophospho-N-acetyl-neuraminic acid (CMP-NANA) and analyzed lipooligosaccharide (LOS) size and monoclonal antibody epitope binding.
Treated modified LOS with neuraminidase and exposed gonococcal strains to human polymorphonuclear leukocytes (PMNs).
Assayed detergent extracts of gonococci with radiolabeled NANA and evaluated exogenous sialyltransferases to test enzymatic LOS modification.
Growth in CMP-NANA increased the molecular mass of the 4.5-kD LOS component by approximately 400 daltons while reducing monoclonal antibody epitope binding, a phenotype fully reversed by neuraminidase treatment.
Incubation of gonococci with human PMNs similarly decreased the surface expression of 4.5-kD LOS epitopes.
Detergent extracts of gonococci incorporated radiolabeled NANA into LOS, demonstrating endogenous sialyltransferase activity alongside susceptibility to exogenous sialyltransferases.

Cite This Study

Mandrell et al. (1990) studied this question.

synapsesocial.com/papers/6a6f85b3e71d69abee0812ec https://doi.org/10.1084/jem.171.5.1649

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Implication

Laboratory study demonstrates sialylation alters Neisseria gonorrhoeae lipooligosaccharide surface epitopes, indicating an endogenous mechanism for immune evasion during infection.

In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.

Discussion

Member takes

Implication

Key Points

Cite This Study

In vitro and in vivo modification of Neisseria gonorrhoeae lipooligosaccharide epitope structure by sialylation.

Discussion

Member takes

Implication

Key Points

Cite This Study

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