What question did this study set out to answer?

To determine how mutations in the HIV-1 Gag p6 domain and its conserved P(T/S)APP motif affect the recruitment and retention of viral Pol proteins during virion assembly.

April 1, 1998Journal of VirologyOpen Access

Mutations of the Human Immunodeficiency Virus Type 1 p6 Gag Domain Result in Reduced Retention of Pol Proteins during Virus Assembly

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Authors

XYXiao-Fang YuBeijing Forestry University LDLiza DawsonWalter Reed Army Institute of Research CTChun-Juan TianJohns Hopkins University

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Implication

In vitro mutational study demonstrates that HIV-1 p6 Gag truncation reduces Pol protein retention in virions, suggesting p6 mediates cleaved enzyme incorporation during assembly.

Key Points

To determine how mutations in the HIV-1 Gag p6 domain and its conserved P(T/S)APP motif affect the recruitment and retention of viral Pol proteins during virion assembly.
Engineered HIV-1 constructs carrying truncations of the C-terminal p6 domain or site-directed mutations within the conserved lentivirus P(T/S)APP motif.
Evaluated the intracellular and virion-associated levels of Gag-Pol precursors and processed Pol proteins in transfected cells, including conditions with an inactivated viral protease.
Truncation of the p6 domain or mutation of the conserved P(T/S)APP motif drastically decreased the retention of mature Pol proteins in released mutant virions.
Intracellular steady-state amounts of Gag-Pol precursor and processed Pol proteins remained unaffected by p6 domain mutations.
Unprocessed Gag-Pol precursors successfully incorporated into p6-mutant virions when viral protease activity was abolished, demonstrating intact Gag and Gag-Pol precursor interactions.

Cite This Study

Yu et al. (1998) studied this question.

synapsesocial.com/papers/6a6f87b331a3df824327e467 https://doi.org/10.1128/jvi.72.4.3412-3417.1998

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Implication

In vitro mutational study demonstrates that HIV-1 p6 Gag truncation reduces Pol protein retention in virions, suggesting p6 mediates cleaved enzyme incorporation during assembly.

Mutations of the Human Immunodeficiency Virus Type 1 p6 Gag Domain Result in Reduced Retention of Pol Proteins during Virus Assembly

Discussion

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Implication

Key Points

Cite This Study

Mutations of the Human Immunodeficiency Virus Type 1 p6 Gag Domain Result in Reduced Retention of Pol Proteins during Virus Assembly

Discussion

Member takes

Implication

Key Points

Cite This Study

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