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High sensitivity of modern detectors enables monitoring and utilisation of very small chromatographic effects caused by pressure. Till now, these effects have been often overlooked. Taking the influence of pressure on the HPLC system into account, numerous unexplained chromatographic phenomenona may be elucidated. Moreover, some pressure effects may be exploited for HPLC column diagnostics, and pressure may even be employed to control retention behaviour of substances. Last but not least, ultrahigh pressure enables transporting eluent through long microparticulate column packings to reach extremely high efficiency of HPLC separations.
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Macko et al. (2001) studied this question.
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