What question did this study set out to answer?

Investigate the binding properties and interactions of the small interstitial proteoglycans decorin, biglycan, and fibromodulin with transforming growth factor beta isoforms.

September 1, 1994Biochemical JournalOpen Access

Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor β

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Authors

AHA. HildebrandOtto-von-Guericke University Magdeburg MRManuel Romarı́sVlaams Instituut voor Biotechnologie LRLars Melholt RasmussenVascular Medicine

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Implication

In vitro study demonstrates that small interstitial proteoglycans bind transforming growth factor beta, suggesting extracellular matrix regulation of growth factor availability.

Key Points

Investigate the binding properties and interactions of the small interstitial proteoglycans decorin, biglycan, and fibromodulin with transforming growth factor beta isoforms.
Expressed human cDNA core proteins of decorin, biglycan, and fibromodulin as maltose-binding fusion proteins and isolated intact proteoglycans from bovine tissues.
Conducted competition binding assays and affinity-labeling experiments using radiolabeled TGF-beta isoforms in Mv 1 Lu epithelial cells and endothelial cells.
Recombinant core proteins demonstrated a two-site binding model for TGF-beta 1, showing Kd values between 1 and 20 nM for a high-affinity site and 50 to 200 nM for a lower-affinity site.
Enzymatic removal of chondroitin and dermatan sulphate chains increased the binding activity of intact decorin and biglycan, showing that glycosaminoglycan chains impede core protein interactions.
Proteoglycan fusion proteins competed with TGF-beta binding to cellular betaglycan, endoglin, and type-I receptors without altering binding to type-II receptors.

Cite This Study

Hildebrand et al. (1994) studied this question.

synapsesocial.com/papers/6a6f8aae75498292b708ebe5 https://doi.org/10.1042/bj3020527

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Implication

In vitro study demonstrates that small interstitial proteoglycans bind transforming growth factor beta, suggesting extracellular matrix regulation of growth factor availability.

Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor β

Discussion

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Implication

Key Points

Cite This Study

Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor β

Discussion

Member takes

Implication

Key Points

Cite This Study

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