What question did this study set out to answer?

To determine the mechanism of cell death triggered by lowering extracellular potassium in cultured cerebellar granule neurons and to evaluate factors that prevent this degeneration.

December 1, 1993Proceedings of the National Academy of SciencesOpen Access

Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP.

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Authors

SDSantosh R. D’MelloItron (United States)CGCinzia GalliInstitute of Neurobiology and Molecular Medicine TCTeresa CiottiInstitute of Cell Biology and Neurobiology

Discussion

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Overview

In vitro study reveals that low potassium induces transcription-dependent apoptosis in cerebellar granule neurons, indicating that IGF-I and cAMP promote neuronal survival.

Key Points

To determine the mechanism of cell death triggered by lowering extracellular potassium in cultured cerebellar granule neurons and to evaluate factors that prevent this degeneration.
Switched differentiated cerebellar granule neuron cultures from high potassium (25 mM) medium to physiological low potassium (5 mM) medium.
Assessed neuronal viability, DNA fragmentation, and the protective effects of transcription and translation inhibitors.
Screened neurotrophic factors and intracellular messengers, including insulin-like growth factor I (IGF-I), cAMP, and phorbol 12-myristate 13-acetate (PMA), for survival-promoting activity.
Shifting to 5 mM potassium triggered apoptotic death characterized by internucleosomal genomic DNA cleavage.
Transcription and translation inhibitors suppressed low-potassium-induced apoptosis, indicating a requirement for newly synthesized gene products.
Insulin-like growth factor I and cAMP prevented apoptosis in low potassium, whereas other growth factors and the protein kinase C activator PMA did not support survival.

Cite This Study

D’Mello et al. (1993) studied this question.

synapsesocial.com/papers/6a6f8e076c240de38cdb83ca https://doi.org/10.1073/pnas.90.23.10989

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December 1, 1993Proceedings of the National Academy of SciencesOpen Access

Induction of apoptosis in cerebellar granule neurons by low potassium: inhibition of death by insulin-like growth factor I and cAMP.

View Full Paper

Ask AI

Bookmark

Authors

SDSantosh R. D’MelloItron (United States)CGCinzia GalliInstitute of Neurobiology and Molecular Medicine TCTeresa CiottiInstitute of Cell Biology and Neurobiology

Discussion

Member takes

Overview

In vitro study reveals that low potassium induces transcription-dependent apoptosis in cerebellar granule neurons, indicating that IGF-I and cAMP promote neuronal survival.

Key Points

To determine the mechanism of cell death triggered by lowering extracellular potassium in cultured cerebellar granule neurons and to evaluate factors that prevent this degeneration.
Switched differentiated cerebellar granule neuron cultures from high potassium (25 mM) medium to physiological low potassium (5 mM) medium.
Assessed neuronal viability, DNA fragmentation, and the protective effects of transcription and translation inhibitors.
Screened neurotrophic factors and intracellular messengers, including insulin-like growth factor I (IGF-I), cAMP, and phorbol 12-myristate 13-acetate (PMA), for survival-promoting activity.
Shifting to 5 mM potassium triggered apoptotic death characterized by internucleosomal genomic DNA cleavage.
Transcription and translation inhibitors suppressed low-potassium-induced apoptosis, indicating a requirement for newly synthesized gene products.
Insulin-like growth factor I and cAMP prevented apoptosis in low potassium, whereas other growth factors and the protein kinase C activator PMA did not support survival.

Cite This Study

D’Mello et al. (1993) studied this question.

synapsesocial.com/papers/6a6f8e076c240de38cdb83ca https://doi.org/10.1073/pnas.90.23.10989

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