Development of a droplet digital PCR assay for detection of group A porcine rotavirus

Figures

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  Figure 1. Influence of annealing temperature on the PoRVA ddPCR system. The assay was conducted across an annealing temperature gradient: 54, 55, 56, 57, 58, 59, and 60°C. NTC, no template control.

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  Figure 2. Influence of primer-to-probe concentration ratio on the PoRVA ddPCR system. The assay was conducted across a primer and probe concentration ratio gradient: 300:200, 800:250, 500:300, and 400:400. NTC, no template control.

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  Figure 3. Quantification of serially diluted PoRVA plasmids by ddPCR and qPCR. (A) Standard curves PoRVA plasmids constructed by ddPCR. The quantification correlation was obtained by plotting the log assumed concentration against the log starting concentration. (B) Standard curves of PoRVA plasmids constructed by qPCR. The quantification correlation was obtained by plotting the quantification cycle value against the log starting concentration.

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  Figure 4. Specificity analysis of the PoRVA ddPCR assay. Lanes 1–8 (divided by vertical black dotted lines): the fluorescence amplitude of PoRVA, TGEV, PEDV, CSFV, PRRSV, PCV2, PRV, and ddH2O, respectively.