What question did this study set out to answer?

Investigate the enzymatic pathways and intermediate products involved in the catabolism and fermentation of L-arginine and L-ornithine by Clostridium botulinum 62-A.

January 1, 1967Journal of BacteriologyOpen Access

Arginine and Ornithine Catabolism by Clostridium botulinum

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Authors

BMB. M. MitrukaWest Virginia University RCRalph N. CostilowAgricultural Research Service

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Implication

Biochemical analysis uncovers arginine and ornithine fermentation pathways in Clostridium botulinum, indicating how anaerobic bacteria utilize single amino acids for energy.

Key Points

Investigate the enzymatic pathways and intermediate products involved in the catabolism and fermentation of L-arginine and L-ornithine by Clostridium botulinum 62-A.
Assayed dihydrolase, arginase, and transamidinase enzymatic activities in cell, spore, and germinated spore extracts of Clostridium botulinum 62-A.
Traced metabolic degradation using radiolabeled substrates (L-ornithine-C14, -1-C14, and -2-C14) in whole cells and cell-free extracts.
Quantified fermentation products, including CO2, NH3, putrescine, delta-aminovaleric acid, and volatile fatty acids to assess carbon and redox balances.
Clostridium botulinum degraded L-arginine to citrulline and ornithine via the arginine dihydrolase system with transamidinase activity, lacking functional arginase.
Cells degraded L-ornithine through partial decarboxylation to putrescine and fermentation to CO2, NH3, delta-aminovalerate, and volatile acids (predominantly acetate, followed by propionate, valerate, and butyrate).
Carbon-14 tracing demonstrated that CO2 originated entirely from carbon 1 and volatile acids from carbons 2 through 5, confirming L-ornithine ferments as a single substrate.

Cite This Study

Mitruka et al. (1967) studied this question.

synapsesocial.com/papers/6a6f93fe439bab0cabc2d8d9 https://doi.org/10.1128/jb.93.1.295-301.1967

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Implication

Biochemical analysis uncovers arginine and ornithine fermentation pathways in Clostridium botulinum, indicating how anaerobic bacteria utilize single amino acids for energy.

Arginine and Ornithine Catabolism by Clostridium botulinum

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Implication

Key Points

Cite This Study

Arginine and Ornithine Catabolism by Clostridium botulinum

Discussion

Member takes

Implication

Key Points

Cite This Study

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