This optimized protocol enables simultaneous dual-channel optical mapping of voltage and calcium in cultured HL-1 atrial myocyte monolayers, providing a valuable tool for studying atrial electrophysiology and arrhythmogenesis.
Provides in vitro tool for atrial electrophysiology research; leaves open translation to intact or clinical models.
Optical mapping has proven to be a valuable technique to detect cardiac electrical activity on both intact ex vivo hearts and in cultured myocyte monolayers. HL-1 cells have been widely used as a 2-Dimensional cellular model for studying diverse aspects of cardiac physiology. However, it has been a great challenge to optically map calcium (Ca) transients and action potentials simultaneously from the same field of view in a cultured HL-1 atrial cell monolayer. This is because special handling and care is required to prepare healthy cells that can be electrically captured and optically mapped. Therefore, we have developed an optimal working protocol for dual channel optical mapping. In this manuscript, we have described in detail how to perform the dual channel optical mapping experiment. This protocol is a useful tool to enhance the understanding of action potential propagation and Ca kinetics in arrhythmia development.
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Yan et al. (2015) studied this question.
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