Accelerating effects of blebbistatin on relaxation process of cell membrane permeabilized trachea and taenia cecum from guinea pig

Figures

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  Fig. 1.. Typical tension traces of relaxation process after Ca2+ removals of β-escin skinned (cell membrane peremiabilized) trachea (A) and taenia cecum (B) preparations in the Relaxing solution (with CTP) with or without blebbistatin at 30 μM, 30.0 ± 1.0 °C. Ordinate: Relative force normalized with the maximal tension level induced by 10-5.0 M Ca2+ promptly before the Ca2+ removal. Abscissa: Time in seonds after the Ca2+ removal.

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  Fig. 2.. Statistical representation for the effects of blebbistatin on the relaxation processes of β-escin skinned tracheal smooth muscle (A) and skinned taenia cecum (B) in the presence of CTP. Control (filled circles), and blebbistatin at 10 μM (open triangles) and 30 μM (filled squares). Symbols and associated bars are means and ± S.E.M., respectively, n=11. *Significant difference of the force compared with that of control, where P<0.05.

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  Fig. 3.. Effects of blebbistatin on the relaxation processes of β-escin skinned tracheal smooth muscle (A) and skinned taenia cecum (B) in the absence of nucleotide (Rigor condition). Control (filled circles), and blebbistatin at 10 μM (open triangles) and 30 μM (filled squares). Symbols and associated bars are means and ± S.E.M., respectively, n=11. *Significant difference from the control (P<0.05).