This report describes the isolation of the natural isomer of 3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid from human bile by a method that retains the configuration at C-25. The stereochemistry at C-25 in this bile acid was defined as 25R by a direct comparison with standard (25R)3 alpha, 7 alpha, 12 alpha-trihydroxy-5 beta-cholestan-26-oic acid.
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Batta et al. (1983) studied this question.
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