The development of an enzyme immunoassay for phospholamban reveals that it and the calcium transporting enzyme are localized at different sites in the cardiac sarcoplasmic reticulum.
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Suggests distinct phospholamban and Ca-ATPase localization in cardiac SR; hypothesis-generating for regulatory mechanisms in mammalian myocardium.
Phospholamban, a phosphorylatable protein of the cardiac sarcoplasmic reticulum, has been estimated by a semi-quantitative immunoassay. It can be determined in purified membrane preparations as well as in crude fractions of cardiac muscle membranes, regardless of the phosphorylation state of the phosphoprotein. The content of phospholamban in mammalian heart muscle membrane vesicles correlates with the activity of the calcium/magnesium-dependent ATPase with the exception of the oxalate-loaded membrane preparations. This observation indicates that phospholamban and the calcium transporting enzyme are localized at different sites in cardiac sarcoplasmic reticulum.
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Martin Holtzhauer (1987) studied this question.
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