Study of the influence of oxo-derivates of nitrogen-containing heterocycles compounds on the intestinal microbiome composition of rats

The effect on the intestinal microbiome of rats of three compounds belonging to different classes of oxo-derivatives of nitrogen-containing heterocycles: CBR-384, CBR-376 and CBR-124 was studied. These substances have high biological activity in animal models and are capable to influence several physiological parameters of Escherichia coli bacteria, which are one of the components of the microbiome of humans and animals. For the first time, a important effect of CBR-384 on the intestinal microbiome composition of rats was shown: a significant decrease in the number of pathogenic bacteria of the genera Shigella and Stenotrophomonas was observed by 10 and 12 times, respectively. In addition, the substance caused an increase in the ratio of the genera Akkermansia / Lachnospiraceae, which, according to literature sources, is a positive change in the microbiome composition. When exposed to CBR-376, there was a 12.9-fold decrease in the number of bacteria from the family Lachnospiraceae and an increase in representatives of the families Xanthomonadaceae (2-fold), Enterobacterales (3-fold) and Pseudomonadaceae (10-fold). CBR-124 did not cause significant changes in the intestinal microbiome composition of rats.