The Potential of Tunable Femtosecond Laser Light to Prevent Melanoma A375 Cell Growth: An In Vitro Investigation

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The incidence and mortality rates associated with melanoma are increasing. Due to their high proliferation rate, ability to self-renew, and resistance mechanisms, cancer cells often withstand conventional therapies such as radiation and chemotherapy. Therefore, further research is required to develop novel melanoma therapies with fewer adverse effects, but effective therapeutic impacts. This study aims to investigate how femtosecond laser treatment affects melanoma cells using the A375 cell line as an in vitro model. A375 melanoma cells were plated at a concentration of 104 cells per well in 96-well plates and incubated overnight; then, they were subjected to femtosecond laser irradiation for durations of 3, 5, or 10 min, maintaining a steady power of 100 mW. The laser operated across different wavelengths in the ultraviolet, visible, and infrared ranges. Cell viability was evaluated 24 h after irradiation using the MTT assay. The results showed the significant inhibition of melanoma cell growth with various femtosecond laser parameters, particularly at 380 and 400 nm. At 380 nm, the cell viability was reduced by approximately 90%, and at 400 nm by 73%, after 10 min of exposure. Additional reductions were observed at 420 nm (47%) and 440 nm (18%), while no significant effects were found at 700–780 nm. The most effective exposure time was 10 min. Femtosecond laser radiation exerts a noteworthy anticancer effect on A375 cells, particularly at specific wavelengths and exposure durations, underscoring the potential of femtosecond laser therapy for treating melanoma. Exploring the underlying mechanisms of these effects and evaluating the clinical potential of this treatment modality requires further research.