What question did this study set out to answer?

Identify the receptor mechanisms responsible for albumin binding and tubular reabsorption in the kidney proximal tubule.

May 15, 2000Journal of Clinical InvestigationOpen Access

Cubilin is an albumin binding protein important for renal tubular albumin reabsorption

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Authors

HBHenrik BirnAarhus University JFJohn C. FyfeMichigan State University CJChristian JacobsenAarhus University

Discussion

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Implication

Experimental study reveals cubilin and megalin mediate renal albumin reabsorption in dogs and mice, suggesting mechanisms for kidney inflammation and fibrosis.

Key Points

Identify the receptor mechanisms responsible for albumin binding and tubular reabsorption in the kidney proximal tubule.
Purified and measured receptor-ligand interactions using affinity chromatography and surface plasmon resonance.
Assessed proximal tubular albumin uptake and urinary excretion in dogs with functional cubilin defects and megalin-deficient mice.
Identified renal albumin-binding protein fragments via immunoblotting and immunocytochemistry.
Identified cubilin as a 460-kDa albumin-binding receptor and revealed that previously identified low-molecular-weight renal albumin-binding proteins are cubilin fragments.
Dogs with functional cubilin defects exhibited virtually abolished proximal tubule reabsorption and excreted large amounts of albumin.
Megalin-deficient mice showed decreased tubular reabsorption and abnormal urinary albumin excretion, supporting a cooperative cubilin-megalin retrieval mechanism.

Cite This Study

Birn et al. (2000) studied this question.

synapsesocial.com/papers/6a6fac08e36a167817e160ff https://doi.org/10.1172/jci8862

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Implication

Experimental study reveals cubilin and megalin mediate renal albumin reabsorption in dogs and mice, suggesting mechanisms for kidney inflammation and fibrosis.

Cubilin is an albumin binding protein important for renal tubular albumin reabsorption

Discussion

Member takes

Implication

Key Points

Cite This Study

Cubilin is an albumin binding protein important for renal tubular albumin reabsorption

Discussion

Member takes

Implication

Key Points

Cite This Study

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