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November 1, 1979Proceedings of the National Academy of SciencesOpen Access

Growth of cultured human epidermal cells into multiple epithelia suitable for grafting.

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Authors

HGH GreenOKO. KehindeJTJudith M. Thomas

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Overview

Experimental study demonstrates rapid expansion of human keratinocytes into stratified epithelia, highlighting potential for autologous grafting.

Key Points

  • To evaluate the feasibility of rapidly growing transplantable, stratified epithelial sheets from isolated human epidermal keratinocyte cultures.
  • Cultured single epidermal keratinocytes obtained from small human skin biopsies under optimized in vitro growth conditions.
  • Monitored individual colonies as they stratified, differentiated, and fused into continuous, cohesive epithelial sheets.
  • Single human keratinocytes successfully formed stratified colonies that fused into an epithelium closely approximating native epidermis.
  • Large surface areas of cultured epithelium were generated rapidly from minimal donor tissue for potential autologous clinical grafting.

Cite This Study

Green et al. (1979) studied this question.

synapsesocial.com/papers/6a6fad99f44fa9f079dd24aehttps://doi.org/10.1073/pnas.76.11.5665
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Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Stratification, specialization, and proliferation of primary keratinocyte cultures. Evidence of a functioning in vitro epidermal cell system.1978 · 181 citations
  2. 2Formation of Epidermis After Reimplantation of Pure Primary Epidermal Cell Cultures From Perinatal Mouse Skin 21974 · 60 citations