Why the study?
Broad genetic diversity detected in TTSuV1 and TTSuVk2 required improved viral genome detection methods to understand their prevalence.
TTSuV1 and TTSuVk2a are widespread in Japanese pig populations, as detected by newly established single PCR-based methods.
Because broad genetic diversity has recently been detected in Torque teno sus viruses (TTSuV1 and TTSuVk2), the viral genome detection method needs to be improved to understand the prevalence of these viruses. Here, we established single PCR-based detection methods for the TTSuV1 and TTSuVk2a genomes with newly designed primer pairs and applied them to investigate the prevalence of TTSuV1 and TTSuVk2a in Japanese pig populations. The results revealed that 98.2% and 81.7% of the pig farms tested positive for the TTSuV1 and TTSuVk2a genomes, respectively, indicating that both TTSuV1 and TTSuVk2a are widespread in Japan.
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Furukawa et al. (2020) studied this question.
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