What question did this study set out to answer?

To determine whether Shiga toxigenic Escherichia coli isolates derived from food and livestock harbor the mucus-activatable stx2d toxin genotype.

August 1, 2003Journal of Clinical MicrobiologyOpen Access

Presence of Activatable Shiga Toxin Genotype ( stx 2d ) in Shiga Toxigenic Escherichia coli from Livestock Sources

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Authors

KGKari S. GobiusCommonwealth Scientific and Industrial Research Organisation GHGlen HiggsCommonwealth Scientific and Industrial Research Organisation PDPatricia DesmarchelierCommonwealth Scientific and Industrial Research Organisation

Discussion

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Implication

Molecular surveillance reveals activatable Shiga toxin genotype stx2d in retail beef isolates, highlighting potential health hazards from meat products.

Key Points

To determine whether Shiga toxigenic Escherichia coli isolates derived from food and livestock harbor the mucus-activatable stx2d toxin genotype.
Screened stx2 operons from food and livestock isolates using PCR-restriction fragment length polymorphism (RFLP) analysis.
Identified candidate activatable stx2d operons by the absence of a PstI restriction site in the stx2A subunit gene.
Confirmed stx2d identity via nucleotide sequencing, verified toxin expression through immunoblotting, and induced bacteriophages carrying stx2d.
Identified 12 STEC isolates carrying putative stx2d operons and determined the complete nucleotide sequences for seven representative operons.
Confirmed Shiga toxin expression by immunoblotting and recovered two related stx2d-carrying bacteriophages (phi1662a and phi1720a) from retail ground beef isolates.

Cite This Study

Gobius et al. (2003) studied this question.

synapsesocial.com/papers/6a6faeefe71d69abee0826ac https://doi.org/10.1128/jcm.41.8.3777-3783.2003

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Implication

Molecular surveillance reveals activatable Shiga toxin genotype stx2d in retail beef isolates, highlighting potential health hazards from meat products.

Presence of Activatable Shiga Toxin Genotype ( stx 2d ) in Shiga Toxigenic Escherichia coli from Livestock Sources

Discussion

Member takes

Implication

Key Points

Cite This Study

Presence of Activatable Shiga Toxin Genotype ( stx 2d ) in Shiga Toxigenic Escherichia coli from Livestock Sources

Discussion

Member takes

Implication

Key Points

Cite This Study

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