What question did this study set out to answer?

To determine whether mouse plasmacytoma cells display surface-bound myeloma proteins identical to their secreted immunoglobulins and assess their viability as immune targets.

August 1, 1972Proceedings of the National Academy of Sciences

Cell-Bound Myeloma Proteins on the Surface of Myeloma Cells: Potential Targets for the Immune System

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Authors

KHKristian HannestadOslo University Hospital MKMing-Shian KaoSaint Louis University HEHerman N. EisenRoche (Switzerland)

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Implication

Experimental study reveals surface-bound myeloma proteins across mouse plasmacytomas, indicating that cell-surface idiotypes can serve as targets for immune recognition.

Key Points

To determine whether mouse plasmacytoma cells display surface-bound myeloma proteins identical to their secreted immunoglobulins and assess their viability as immune targets.
Assayed eleven mouse plasmacytoma lines for surface myeloma proteins using rosette formation with 2,4,6-trinitrophenylated sheep erythrocytes, applied directly or through bispecific hybrid antibody fragments.
Analyzed the heavy and light chains, idiotypic determinants, and ligand-binding specificities of cell-bound immunoglobulins relative to secreted myeloma proteins.
Isolated and characterized a variant line (MOC-315 NR) derived from non-rosetting MOPC-315 cells.
Cell-bound myeloma proteins were detected across all eleven mouse plasmacytomas tested, with MOPC-315 and MOPC-460 binding target erythrocytes directly and nine others requiring hybrid antibody fragments.
Surface-bound immunoglobulins on MOPC-315 and MOPC-460 possessed the identical heavy chains, light chains, idiotypic determinants, and ligand affinities as their secreted counterparts.
The variant tumor MOC-315 NR arose from the 5% to 10% of non-binding MOPC-315 cells, expressing and secreting only the light chain in a pattern consistent with in vivo immunoselection.

Cite This Study

Hannestad et al. (1972) studied this question.

synapsesocial.com/papers/6a6fb09f35aa2c282ce1076e https://doi.org/10.1073/pnas.69.8.2295

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Implication

Experimental study reveals surface-bound myeloma proteins across mouse plasmacytomas, indicating that cell-surface idiotypes can serve as targets for immune recognition.

Cell-Bound Myeloma Proteins on the Surface of Myeloma Cells: Potential Targets for the Immune System

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Implication

Key Points

Cite This Study

Cell-Bound Myeloma Proteins on the Surface of Myeloma Cells: Potential Targets for the Immune System

Discussion

Member takes

Implication

Key Points

Cite This Study

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