Evidence for the phosphorylation of enzyme IIglucose of the phosphoenolpyruvate—sugar phosphotransferase system of Escherichia coli and Salmonella typhimurium

A membrane‐bound phosphoprotein, of subunit Mr 48 000 by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis, has been found in Escherichia coli and Salmonella typhimurium . The phosphorylation of this protein is dependent upon phospho enol pyruvate and the protein of the sugar phosphotransferase (PT) system: enzyme I, HPr, and III glucose . The membrane‐bound phosphoprotein is identified as enzyme II glucose . Other membrane‐bound phosphoproteins, that are also dependent upon the PT system, remain to be identified.