September 1, 1993Journal of Clinical MicrobiologyOpen Access

Detection of rotavirus serotypes G1, G2, G3, and G11 in feces of diarrheic calves by using polymerase chain reaction-derived cDNA probes

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Population

59 bovine rotavirus strains that were nonreactive with cDNA probes to G6, G8, and G10

Design

Preclinical

Authors

AHAhmed A. HusseinDirectorate of Health APAnil V. ParwaniNorthwestern University BRB I RosenNew York State Department of Health

Discussion

Member takes

Overview

First US bovine rotavirus serotypes reported in calves; extends surveillance data but leaves diagnostic and vaccine implications open.

Structured PICO

Population

59 bovine rotavirus (BRV) strains (fecal samples from diarrheic calves in Ohio, Nebraska, Washington, and South Dakota) that were nonreactive with cDNA probes to G6, G8, and G10

Intervention

cDNA probes prepared by polymerase chain reaction amplification of the hyperdivergent regions of the VP7 genes from human (G1, G2, and G3) and porcine (G4, G5, and G11) rotaviruses used in a dot blot hybridization assay

Outcome

Identification of rotavirus serotypes G1, G2, G3, and G11 among the BRV field samplessurrogate

This study provides the first report in the United States of the identification of rotavirus serotypes G1, G2, G3, and G11 among bovine rotavirus isolates from diarrheic calves.

Cite This Study

Hussein et al. (1993) studied this question.

synapsesocial.com/papers/6a6fb25ca2d7cf2e39c294a6 https://doi.org/10.1128/jcm.31.9.2491-2496.1993

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September 1, 1993Journal of Clinical MicrobiologyOpen Access

Detection of rotavirus serotypes G1, G2, G3, and G11 in feces of diarrheic calves by using polymerase chain reaction-derived cDNA probes

View Full Paper

Ask AI

Bookmark

Population

59 bovine rotavirus strains that were nonreactive with cDNA probes to G6, G8, and G10

Design

Preclinical

Authors

AHAhmed A. HusseinDirectorate of Health APAnil V. ParwaniNorthwestern University BRB I RosenNew York State Department of Health

Discussion

Member takes

Overview

First US bovine rotavirus serotypes reported in calves; extends surveillance data but leaves diagnostic and vaccine implications open.

Structured PICO

Population

59 bovine rotavirus (BRV) strains (fecal samples from diarrheic calves in Ohio, Nebraska, Washington, and South Dakota) that were nonreactive with cDNA probes to G6, G8, and G10

Intervention

Outcome

Identification of rotavirus serotypes G1, G2, G3, and G11 among the BRV field samplessurrogate

This study provides the first report in the United States of the identification of rotavirus serotypes G1, G2, G3, and G11 among bovine rotavirus isolates from diarrheic calves.

Cite This Study

Hussein et al. (1993) studied this question.

synapsesocial.com/papers/6a6fb25ca2d7cf2e39c294a6 https://doi.org/10.1128/jcm.31.9.2491-2496.1993

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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