January 1, 1989Journal of Clinical MicrobiologyOpen Access

Dot hybridization assay for distinction of rotavirus serotypes

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Why the study?

Does a dot hybridization assay accurately distinguish rotavirus serotypes compared to a monoclonal antibody assay?

Population

24 laboratory specimens and 103 clinical specimens of rotavirus from Washington, D.C., Venezuela, and Chile

Comparison

Dot hybridization assay using 32P-labeled cDNA… vs Monoclonal antibody serotyping assay

Design

Preclinical

Authors

JFJorge FloresProgram for Appropriate Technology in Health KGK Y GreenNational Institutes of Health DGD GarciaScripps Research Institute

Discussion

Member takes

Implication

Offers alternative to monoclonal antibody serotyping in animal models; leaves open human validation and clinical adoption.

Structured PICO

Does a dot hybridization assay accurately distinguish rotavirus serotypes compared to a monoclonal antibody assay?

Population

24 laboratory specimens and 103 clinical specimens of rotavirus from Washington, D.C., Venezuela, and Chile

Intervention

Dot hybridization assay using 32P-labeled cDNA probes

Comparator

Monoclonal antibody serotyping assay

Outcome

Distinction of rotavirus serotypes 1, 2, 3, and 4surrogate

A newly developed dot hybridization assay can accurately distinguish rotavirus serotypes 1-4, performing similarly to monoclonal antibody assays.

Cite This Study

Flores et al. (1989) studied this question.

synapsesocial.com/papers/6a6fb294439bab0cabc2e160 https://doi.org/10.1128/jcm.27.1.29-34.1989

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Dot hybridization assay for distinction of rotavirus serotypes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Dot hybridization assay for distinction of rotavirus serotypes

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider