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June 1, 1985Journal of VirologyOpen Access

Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis

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Population

Rotavirus strains S2 (human serotype 2) and Nebraska calf diarrhea virus (bovine serotype)

Design

Preclinical

Authors

PGPeter GunnUniversity of Technology SydneyFSFumihiko SatoKyoto UniversityKPK.F.H. PowellAuckland City Hospital

Discussion

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Overview

Linear VP7 peptides fail to elicit neutralizing antisera; hypothesis-generating for conformational epitope strategies in rotavirus vaccine research.

Structured PICO

P
Population
Rotavirus strains S2 (human serotype 2) and Nebraska calf diarrhea virus (bovine serotype)
I
Intervention
Synthesis of six peptides spanning hydrophilic regions of VP7 and generation of antisera
O
Outcome
Recognition of homologous peptides, binding to denatured VP7, recognition of virus, and neutralizing activitysurrogate

Synthesized peptides spanning hydrophilic regions of rotavirus VP7 generated antisera that bound denatured protein but failed to neutralize the virus, suggesting these sequences are not exposed on the viral surface.

Cite This Study

Gunn et al. (1985) studied this question.

synapsesocial.com/papers/6a6fb3118031ec7bb1dbe5abhttps://doi.org/10.1128/jvi.54.3.791-797.1985
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