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March 1, 1980Journal of VirologyOpen Access

In vitro transcription and translation of simian rotavirus SA11 gene products

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Authors

BMB B MasonCincinnati Children's Hospital Medical CenterDGDavid Y. GrahamUniversità Cattolica del Sacro CuoreMEM K EstesBaylor College of Medicine

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Implication

Randomized trial examines viral RNA products in simian rotavirus, revealing key polypeptides and structures.

Key Points

  • The study aims to analyze the gene products of simian rotavirus SA11 using in vitro transcription and translation methods.
  • Utilized RNA-dependent RNA polymerase for synthesizing viral RNA transcripts from simian rotavirus SA11.
  • Conducted sedimentation velocity sucrose gradient analysis and agarose gel electrophoresis to evaluate RNA transcripts.
  • Translated RNA products in a mRNA-dependent cell-free system using rabbit reticulocytes.
  • Identified four peaks of RNA transcripts at 9S, 12S, 14S, and 18S, with molecular weights from 2 x 10^5 to 1.2 x 10^6.
  • Synthesized 11 polypeptides with molecular weights ranging from 125,000 to 20,000; three key structural proteins were not synthesized as primary products.
  • Determined some polypeptides as precursors to viral glycoproteins or nonstructural polypeptides.

Cite This Study

Mason et al. (1980) studied this question.

synapsesocial.com/papers/6a6fb313fe4101aa97dfdfbehttps://doi.org/10.1128/jvi.33.3.1111-1121.1980
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Also Consider

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  1. 1Polypeptides of Bovine Rotavirus1979 · 34 citations
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