What question did this study set out to answer?

To identify novel genes selectively induced or repressed by physiological concentrations of interferon-beta in primary peripheral blood mononuclear cells.

August 20, 2007Journal of Leukocyte Biology

Novel interferon-β-induced gene expression in peripheral blood cells

View Full Paper

Ask AI

Bookmark

Authors

MRM.R. Sandhya RaniThe University of Texas Health Science Center at Houston JSJennifer ShrockCleveland Clinic Lerner College of Medicine SASwathi AppachiCleveland Clinic

Discussion

Member takes

Implication

Gene expression profiling reveals novel interferon-stimulated and repressed genes in peripheral blood cells, suggesting improved targets for monitoring therapeutic response.

Key Points

To identify novel genes selectively induced or repressed by physiological concentrations of interferon-beta in primary peripheral blood mononuclear cells.
Exposed whole blood to physiological concentrations of interferon-beta, isolated peripheral blood mononuclear cells (PBMC), and performed cDNA microarray hybridization.
Validated candidate gene regulation using polymerase chain reaction in PBMC, astrocytoma cells, and ex vivo blood samples from patients injected with interferon-beta.
Identified 39 interferon-stimulated genes, including six novel genes: GTP cyclohydrolase 1, LOC129607, FLJ38348, leucine aminopeptidase 3, squalene epoxidase, and a muscle-associated GTP-binding protein.
Identified 20 interferon-repressed genes, confirming down-regulation of IL-1beta and CXCL8 while discovering CXCL1 as a novel repressed gene.

Cite This Study

Rani et al. (2007) studied this question.

synapsesocial.com/papers/6a6fbd0ce71d69abee082f44 https://doi.org/10.1189/jlb.0507273

View Full Paper

Ask AI

Bookmark

Implication

Gene expression profiling reveals novel interferon-stimulated and repressed genes in peripheral blood cells, suggesting improved targets for monitoring therapeutic response.

Novel interferon-β-induced gene expression in peripheral blood cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Novel interferon-β-induced gene expression in peripheral blood cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Also Consider

Also Consider