The 5' and 3' untranslated regions of satellite tobacco necrosis virus RNA affect translational efficiency and dependence on a 5' cap structure

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Population

In vitro translation system from wheat germ using satellite tobacco necrosis virus RNA and chimeric mRNAs

Comparison

Mutations in the 5' and 3' UTRs of STNV RNA… vs Wild-type STNV RNA or uncapped mutant mRNAs

Design

Preclinical

Authors

Discussion

Remains preclinical; leaves open whether UTR interactions govern cap-independent translation in mammalian systems.

Population

In vitro translation system from wheat germ using satellite tobacco necrosis virus RNA (STNV RNA) and chimeric mRNAs

Intervention

Mutations in the 5' and 3' UTRs of STNV RNA, capping of mutant mRNAs, and construction of chimeric mRNAs

Comparator

Wild-type STNV RNA or uncapped mutant mRNAs

Outcome

Translational efficiency and cap independencesurrogate

Interaction between the 5' UTR and the region between nucleotides 627 and 737 in the 3' UTR of STNV RNA is required for cap-independent translation.

Timmer et al. (1993) studied this question.

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