Detection of Numerous Y Chromosome Biallelic Polymorphisms by Denaturing High-Performance Liquid Chromatography
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Implication
Genetic analysis reveals 19 novel Y chromosome biallelic polymorphisms across diverse global populations, indicating ancient bottleneck events and expanding human genealogical resolution.
Key Points
- To evaluate the efficacy of denaturing high-performance liquid chromatography (DHPLC) for the rapid, automated discovery and typing of human Y chromosome biallelic polymorphisms to reconstruct human evolutionary history.
- Utilized denaturing high-performance liquid chromatography (DHPLC) for automated, sensitive heteroduplex detection in amplified Y chromosome sequences.
- Determined the allelic states of 22 Y chromosome polymorphisms across 718 diverse extant chromosomes to establish haplotype frequencies and deduce phylogenetic relationships.
- Discovered and characterized 22 Y chromosome polymorphisms, 19 of which were previously unreported, with most markers showing regionally restricted distribution alongside core, widely dispersed haplotypes.
- Identified a specific transversion defining a major haplogroup that marks a deep, non-African branch, providing evidence for an ancient population bottleneck.
Cite This Study
Underhill et al. (1997) studied this question.