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A 1.6 kb fragment, which contains complete sequence of NIb gene of a soil-borne wheat bymovirus, collected from infected field in Pujiang, Zhejiang Province, was amplified by RT-PCR with a pair of primers (NIb01 and NIb02), which were designed according to the published sequences of Japanese wheat yellow mosaic bymovirus (WYMV), cloned and sequenced. The sequence comparison indicated that the homology of this NIb gene to Japanese WYMV isolates was very high (99.1% identity in nucleotides), but the homology with other bymoviruses was 73.1%-74.4% (nucleotides). The results confirmed that the virus in Pujiang was indeed wheat yellow mosaic virus (WYMV), rather than WSSMV.
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Devine et al. (1977) studied this question.
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