SUMMARY. A method has been established to estimate semiquantitatively the activity of HSD enzymes and is described in detail. In the main, the method depends on demonstrating NAD‐dependent hydroxysteroid dehydrogenase by using nitro‐BT as a hydrogen acceptor leading to the deposition of blue diformazan particles. The latter are then counted in a sebaceous gland by means of a Wild M20 microscope with a drawing tube attachment. The method is limited in use as a semi‐quantitative comparison of enzyme activity in a few adjacent sections of a sebaceous gland.
No takes yet. Share an insight, caveat, or question.
Muir et al. (1968) studied this question.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: