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The pH of mitochondria (pHm) has a close relationship with many biological processes. Here we developed a new indicator Mito-pH-1 for the ratiometric fluorescence detection of the mitochondria pH value, which has excellent tolerance to environmental change. And Mito-pH-1 has been used for the first time to monitor the change of pHm under temperature and H2O2 stimuli in living cells.
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Cao et al. (2015) studied this question.
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