Summary: A method is described whereby pH and Eh of a medium can be closely controlled during the growth of Clostridium welchii type A for long enough to study the bacteriostatic effects of horse antiserum. The pH value was controlled manually to about ± 0·01 unit (s.d.), by adjustments in the CO2 tension of the gas above the medium. The Eh was controlled by the addition of oxygen to a system with an inherent tendency to become reduced. With manual control the Eh was maintained within ± 2 mV (s.d.); automatic control gave better regulation than this.
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Dobson et al. (1964) studied this question.
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