What question did this study set out to answer?

Determine whether the conserved Arabidopsis UPF3 gene functions within the nonsense-mediated mRNA decay pathway to eliminate aberrantly spliced transcripts bearing premature termination codons.

July 7, 2005The Plant JournalOpen Access

UPF3 suppresses aberrant spliced mRNA in Arabidopsis

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Authors

KHKoichi HoriTokyo Institute of Technology YWYuichiro WatanabeTokyo University of the Arts

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Implication

Genetic analysis demonstrates elevated aberrantly spliced transcripts in upf3 mutant Arabidopsis, indicating that AtUPF3 is essential for plant nonsense-mediated mRNA decay.

Key Points

Determine whether the conserved Arabidopsis UPF3 gene functions within the nonsense-mediated mRNA decay pathway to eliminate aberrantly spliced transcripts bearing premature termination codons.
Queried The Arabidopsis Information Resource database to identify candidate genes producing mRNAs with premature termination codons.
Selected five genes, confirmed the in vivo presence of premature termination codons generated via alternative splicing, and compared the ratio of premature-codon-positive to premature-codon-negative transcripts between wild-type and atupf3 mutant Arabidopsis plants.
Identified alternative splice variants containing premature termination codons (PTC+) and variants lacking them (PTC-) across all five selected genes.
Demonstrated higher PTC+/PTC- transcript ratios in atupf3 mutants relative to wild-type plants, reflecting an impaired capacity to degrade premature-codon-bearing mRNAs in the absence of functional AtUPF3.

Cite This Study

Hori et al. (2005) studied this question.

synapsesocial.com/papers/6a6fd78df44fa9f079dd5580 https://doi.org/10.1111/j.1365-313x.2005.02473.x

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Implication

Genetic analysis demonstrates elevated aberrantly spliced transcripts in upf3 mutant Arabidopsis, indicating that AtUPF3 is essential for plant nonsense-mediated mRNA decay.

UPF3 suppresses aberrant spliced mRNA in Arabidopsis

Discussion

Member takes

Implication

Key Points

Cite This Study

UPF3 suppresses aberrant spliced mRNA in Arabidopsis

Discussion

Member takes

Implication

Key Points

Cite This Study

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