What question did this study set out to answer?

To determine whether core histones and native nucleosomes act as glutaminyl substrates for tissue transglutaminase and identify specific modified residues.

August 1, 1996Journal of Biological ChemistryOpen Access

Core Histones Are Glutaminyl Substrates for Tissue Transglutaminase

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Authors

EBEsteban BallestarJosep Carreras Leukaemia Research Institute CAConcepción AbadUniversitat de València LFLuís FrancoUniversitat de València

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Implication

In vitro study reveals specific glutamine modification of core histones by tissue transglutaminase, suggesting potential roles in chromatin regulation.

Key Points

To determine whether core histones and native nucleosomes act as glutaminyl substrates for tissue transglutaminase and identify specific modified residues.
Incubated chicken erythrocyte core histones and native nucleosomes with tissue transglutaminase in the presence of monodansylcadaverine, methylamine, or putrescine as donor amines.
Evaluated histone structural stability and nucleosome reconstitution after modification using circular dichroism spectroscopy.
Nine of 18 glutamines across core histones act as amine acceptors in free histones, undergoing modification much faster than typical native transglutaminase substrates.
Reactions on native nucleosomes restrict modification to H3 Gln5, H3 Gln19, and H2B Gln22 without altering histone secondary structure or core particle assembly.

Cite This Study

Ballestar et al. (1996) studied this question.

synapsesocial.com/papers/6a6fd9bf35aa2c282ce12bd5 https://doi.org/10.1074/jbc.271.31.18817

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Implication

In vitro study reveals specific glutamine modification of core histones by tissue transglutaminase, suggesting potential roles in chromatin regulation.

Core Histones Are Glutaminyl Substrates for Tissue Transglutaminase

Discussion

Member takes

Implication

Key Points

Cite This Study

Core Histones Are Glutaminyl Substrates for Tissue Transglutaminase

Discussion

Member takes

Implication

Key Points

Cite This Study

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