Methods for the immunoassay of blood insulin by double antibody techniques have recently been devised by Hales & Randle (1963) and Morgan & Lazarow (1963). The first of these methods has proved extremely satisfactory, and has in general given values for serum insulin which are in close agreement with those obtained by most other immunoassay procedures. However, using method C of Hales & Randle on sera from twenty-three normal, non-obese subjects, with no family history of diabetes mellitus, excessively high values for serum insulin were found repeatedly in three of the subjects, both in the fasting state and after glucose. It seemed possible that the reason for these high values might lie in the presence in their serum of a factor, similar to that described by Morgan, Sorensen & Lazarow (1964) in rat plasma, which may inhibit the precipitation of the insulin-antibody complex. It has been suggested by Morgan et
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Sheldon et al. (1965) studied this question.