What question did this study set out to answer?

To investigate the receptor-mediated signaling pathways coupling agonist stimulation to cytosolic free calcium elevation, shape alteration, and peroxidase exocytosis in human eosinophils.

June 1, 1991Journal of Clinical InvestigationOpen Access

Shape changes, exocytosis, and cytosolic free calcium changes in stimulated human eosinophils.

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To investigate the receptor-mediated signaling pathways coupling agonist stimulation to cytosolic free calcium elevation, shape alteration, and peroxidase exocytosis in human eosinophils.
Stimulated purified normal-density eosinophils from patients with hypereosinophilic syndrome with C5a, PAF, FMLP, or NAP-1/IL-8.
Quantified cytosolic free calcium with indo-1 fluorescence, shape alterations by laser turbidimetry, and eosinophil peroxidase release via chemiluminescence.
Evaluated signaling cascades using pertussis toxin pretreatment, intracellular calcium depletion, and protein kinase inhibition with staurosporine.
C5a and PAF induced potent calcium mobilization, shape transformation, and eosinophil peroxidase release, whereas FMLP and NAP-1/IL-8 stimulated only minor responses.
Pertussis toxin attenuated calcium influx and peroxidase release, with weaker inhibition of shape change, demonstrating the involvement of Gi-type G-proteins.
Shape changes proceeded independently of calcium depletion or staurosporine, while exocytosis was fully abolished by calcium depletion and markedly augmented by staurosporine.

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Implication

Experimental study reveals distinct activation mechanisms for shape change and exocytosis in human eosinophils, indicating motility does not require classical calcium signaling.

Cite This Study

Kernen et al. (1991) studied this question.

synapsesocial.com/papers/6a6fe1c678a11c550e09a986 https://doi.org/10.1172/jci115230

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Implication

Experimental study reveals distinct activation mechanisms for shape change and exocytosis in human eosinophils, indicating motility does not require classical calcium signaling.

Shape changes, exocytosis, and cytosolic free calcium changes in stimulated human eosinophils.

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Discussion

Implication

Cite This Study

Shape changes, exocytosis, and cytosolic free calcium changes in stimulated human eosinophils.

Key Points

Discussion

Implication

Cite This Study

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