What question did this study set out to answer?

To investigate the role of sialoglycans on lymphatic endothelial cells in mediating interactions with Siglec-1-expressing macrophages in lymph nodes.

October 26, 2021The FASEB JournalOpen Access

Sialoglycans on lymphatic endothelial cells augment interactions with Siglec‐1 (CD169) of lymph node macrophages

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In vitro and mouse study shows lymphatic endothelial sialoglycans bind macrophage Siglec-1, indicating an essential anchoring niche for immune cells.

To investigate the role of sialoglycans on lymphatic endothelial cells in mediating interactions with Siglec-1-expressing macrophages in lymph nodes.
Analyzed glycan profiles on lymphatic and blood endothelial cells from human and murine tissues using lectin-binding assays and mass spectrometry.
Evaluated Siglec-1 binding affinity to lymph node lymphatic endothelial cells and assessed macrophage localization and phenotype in mice expressing a binding-defective Siglec-1 mutant.
Lymphatic endothelial cells in human skin, as well as murine skin, lung, and lymph nodes, displayed higher levels of sialylated and sulfated glycans than blood endothelial cells, with the highest densities on subcapsular sinus floor cells.
Siglec-1 bound strongly to lymphatic endothelial sialoglycans, and mice carrying a binding-defective Siglec-1 mutant showed significantly reduced numbers of subcapsular sinus macrophages.
Residual Siglec-1-positive macrophages in mutant mice displayed diminished proliferative capacity and shifted toward an anti-inflammatory phenotype.

D’Addio et al. (2021) studied this question.

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In vitro and mouse study shows lymphatic endothelial sialoglycans bind macrophage Siglec-1, indicating an essential anchoring niche for immune cells.