What question did this study set out to answer?

To determine the specific structural domains within SAP97 responsible for its recruitment and cortical cytoskeletal attachment at the epithelial lateral membrane.

August 15, 1998Journal of Cell Science

Subcellular targeting and cytoskeletal attachment of SAP97 to the epithelial lateral membrane

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Authors

HWHongju WuTulane University SRSusanne M. ReuverUniversity of Alabama at Birmingham SKSven KuhlendahlUniversity of Alabama at Birmingham

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Implication

Experimental study demonstrates that the N-terminal sequence directs SAP97 localization to lateral membranes in epithelial cells, highlighting domain-specific cytoskeletal anchoring mechanisms.

Key Points

To determine the specific structural domains within SAP97 responsible for its recruitment and cortical cytoskeletal attachment at the epithelial lateral membrane.
Evaluated individual domains of SAP97, including PDZ, SH3, GK, and specific insert sequences, in epithelial cell models.
Assessed the requirement of specific protein regions for selective recruitment and attachment to the cortical cytoskeleton at sites of cell-cell contact.
The PDZ, SH3, and GK domains, as well as the I3 insert, were not required for lateral membrane targeting, though PDZ1-2 and the I3 insert modulated localization efficiency.
The unique N-terminal 65 amino acid residues directed selective subcellular localization and mediated attachment to the cortical cytoskeleton at cell-cell contact sites.

Cite This Study

Wu et al. (1998) studied this question.

synapsesocial.com/papers/6a6fe43b75498292b709447c https://doi.org/10.1242/jcs.111.16.2365

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Implication

Experimental study demonstrates that the N-terminal sequence directs SAP97 localization to lateral membranes in epithelial cells, highlighting domain-specific cytoskeletal anchoring mechanisms.

Subcellular targeting and cytoskeletal attachment of SAP97 to the epithelial lateral membrane

Discussion

Member takes

Implication

Key Points

Cite This Study

Subcellular targeting and cytoskeletal attachment of SAP97 to the epithelial lateral membrane

Discussion

Member takes

Implication

Key Points

Cite This Study

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