What question did this study set out to answer?

To develop and evaluate a multiplex PCR assay targeting 16S rRNA and sodA genes for simultaneous genus- and species-level identification of enterococci.

August 1, 2004Journal of Clinical MicrobiologyOpen Access

Use of a Genus- and Species-Specific Multiplex PCR for Identification of Enterococci

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To develop and evaluate a multiplex PCR assay targeting 16S rRNA and sodA genes for simultaneous genus- and species-level identification of enterococci.
Combined Enterococcus genus-specific 16S rRNA primers with species-specific superoxide dismutase (sodA) primers across seven multiplex PCR mixtures to detect 23 Enterococcus species.
Evaluated isolates from swine feces, poultry carcasses, environmental sources, and retail food against standard biochemical testing, VITEK, API Rapid ID 32 Strep, and BBL Crystal kits.
Multiplex PCR identification agreed with standard biochemical testing and VITEK in 90% of tested isolates.
PCR results concurred with API Rapid ID 32 Strep in 85% of isolates and BBL Crystal in 82% of isolates, matching or exceeding concordance between conventional phenotypic tests.

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Implication

Diagnostic study reveals high agreement between multiplex PCR and standard methods for identifying Enterococcus species in animal and environmental isolates, highlighting an improved rapid assay.

Cite This Study

Jackson et al. (2004) studied this question.

synapsesocial.com/papers/6a6fe84bf44fa9f079dd66f3 https://doi.org/10.1128/jcm.42.8.3558-3565.2004

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Implication

Diagnostic study reveals high agreement between multiplex PCR and standard methods for identifying Enterococcus species in animal and environmental isolates, highlighting an improved rapid assay.

Use of a Genus- and Species-Specific Multiplex PCR for Identification of Enterococci

Key Points

Discussion

Implication

Cite This Study

Use of a Genus- and Species-Specific Multiplex PCR for Identification of Enterococci

Key Points

Discussion

Implication

Cite This Study

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