What question did this study set out to answer?

To develop and evaluate a rapid, sensitive spectrophotometric technique for quantifying carboxyhaemoglobin concentrations from minimal capillary blood volumes.

April 1, 1965Occupational and Environmental MedicineOpen Access

A Sensitive Method for the Determination of Carboxyhaemoglobin in a Finger Prick Sample of Blood

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Authors

BCB. T. ComminsSt Bartholomew's Hospital PLP. J. LawtherPace University

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Implication

Method development study demonstrates rapid spectrophotometric measurement of carboxyhaemoglobin in capillary blood, highlighting a sensitive tool for detecting low-level carbon monoxide exposure.

Key Points

To develop and evaluate a rapid, sensitive spectrophotometric technique for quantifying carboxyhaemoglobin concentrations from minimal capillary blood volumes.
Diluted 0.01 mL of finger-prick blood in 10 mL of 0.04% ammonia solution and divided the mixture into two aliquots.
Bubbled oxygen through one aliquot to convert carboxyhaemoglobin to oxyhaemoglobin, then measured and compared the absorption spectra of both aliquots using a spectrophotometer.
Calculated carboxyhaemoglobin levels graphically or through a standard mathematical formula after a single initial calibration.
Total assay completion required approximately 20 minutes per sample, including blood collection time.
The analytical assay demonstrated high sensitivity, reliably measuring carboxyhaemoglobin concentrations below 1% in blood solutions.

Cite This Study

Commins et al. (1965) studied this question.

synapsesocial.com/papers/6a6fe9e478a11c550e09b18d https://doi.org/10.1136/oem.22.2.139

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Implication

Method development study demonstrates rapid spectrophotometric measurement of carboxyhaemoglobin in capillary blood, highlighting a sensitive tool for detecting low-level carbon monoxide exposure.

A Sensitive Method for the Determination of Carboxyhaemoglobin in a Finger Prick Sample of Blood

Discussion

Member takes

Implication

Key Points

Cite This Study

A Sensitive Method for the Determination of Carboxyhaemoglobin in a Finger Prick Sample of Blood

Discussion

Member takes

Implication

Key Points

Cite This Study

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