What question did this study set out to answer?

To identify the specific nitric oxide synthase isoform responsible for endogenous nitric oxide synthesis in normal human airway epithelium in vivo.

August 15, 1995Proceedings of the National Academy of SciencesOpen Access

Continuous nitric oxide synthesis by inducible nitric oxide synthase in normal human airway epithelium in vivo.

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Authors

FGFangyang GuoJiangxi University of Traditional Chinese Medicine HRH. R. De RaeveAntwerp University Hospital TRThomas W. RiceUniversity of California, Los Angeles

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Overview

In vivo study reveals continuous inducible nitric oxide synthase expression in human airway epithelium, highlighting epithelial cells' active role in respiratory host defense and inflammation.

Key Points

To identify the specific nitric oxide synthase isoform responsible for endogenous nitric oxide synthesis in normal human airway epithelium in vivo.
Cloned and characterized nitric oxide synthase (NOS) expression across human pulmonary cell types using molecular cloning and in situ analysis.
Measured NOS activity in epithelial cell lysates in vivo, assessed expression stability ex vivo, and evaluated the inhibitory effects of inhaled corticosteroids and beta-adrenergic agonists.
Normal human airway epithelial cells continuously expressed inducible NOS (iNOS) mRNA and produced nanomolar concentrations of nitric oxide in vivo, whereas other pulmonary cell types showed no detectable expression.
Removing epithelial cells from the in vivo airway microenvironment resulted in a rapid loss of iNOS expression.
Treatment with inhaled corticosteroids and beta-adrenergic agonists markedly reduced iNOS expression in the airway epithelium.

Cite This Study

Guo et al. (1995) studied this question.

synapsesocial.com/papers/6a6feb4fc2d7c3090826d3c3 https://doi.org/10.1073/pnas.92.17.7809

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August 15, 1995Proceedings of the National Academy of SciencesOpen Access

Continuous nitric oxide synthesis by inducible nitric oxide synthase in normal human airway epithelium in vivo.

View Full Paper

Ask AI

Bookmark

Authors

FGFangyang GuoJiangxi University of Traditional Chinese Medicine HRH. R. De RaeveAntwerp University Hospital TRThomas W. RiceUniversity of California, Los Angeles

Discussion

Member takes

Overview

In vivo study reveals continuous inducible nitric oxide synthase expression in human airway epithelium, highlighting epithelial cells' active role in respiratory host defense and inflammation.

Key Points

To identify the specific nitric oxide synthase isoform responsible for endogenous nitric oxide synthesis in normal human airway epithelium in vivo.
Cloned and characterized nitric oxide synthase (NOS) expression across human pulmonary cell types using molecular cloning and in situ analysis.
Measured NOS activity in epithelial cell lysates in vivo, assessed expression stability ex vivo, and evaluated the inhibitory effects of inhaled corticosteroids and beta-adrenergic agonists.
Normal human airway epithelial cells continuously expressed inducible NOS (iNOS) mRNA and produced nanomolar concentrations of nitric oxide in vivo, whereas other pulmonary cell types showed no detectable expression.
Removing epithelial cells from the in vivo airway microenvironment resulted in a rapid loss of iNOS expression.
Treatment with inhaled corticosteroids and beta-adrenergic agonists markedly reduced iNOS expression in the airway epithelium.

Cite This Study

Guo et al. (1995) studied this question.

synapsesocial.com/papers/6a6feb4fc2d7c3090826d3c3 https://doi.org/10.1073/pnas.92.17.7809

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