What question did this study set out to answer?

To determine the expression patterns of class I homeobox (Hox) genes in functionally distinct CD34+ stem and progenitor cell subpopulations from normal human bone marrow.

December 6, 1994Proceedings of the National Academy of SciencesOpen Access

Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells.

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Authors

GSGuy SauvageauHôpital Maisonneuve-Rosemont PLPeter M. LansdorpBroad Institute CEConnie J. EavesAtrium Health Wake Forest Baptist

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Implication

Gene expression profiling reveals distinct homeobox gene patterns in human bone marrow CD34+ subpopulations, indicating a regulated developmental program in primitive blood cells.

Key Points

To determine the expression patterns of class I homeobox (Hox) genes in functionally distinct CD34+ stem and progenitor cell subpopulations from normal human bone marrow.
Purified functionally distinct CD34+ stem and progenitor cell subsets alongside CD34- cells from normal human bone marrow.
Analyzed class I Hox gene expression using a PCR-based total cDNA amplification technique designed for limited cell quantities.
Identified the expression of at least 16 different Hox genes, predominantly from the A and B clusters, across hematopoietic cell subpopulations.
Detected marked elevation of 3' cluster genes (such as HoxB3) specifically in the most primitive CD34+ subpopulations, whereas 5' cluster genes (such as HoxA10) were expressed uniformly across CD34+ subsets and both were extinguished in CD34- cells.

Cite This Study

Sauvageau et al. (1994) studied this question.

synapsesocial.com/papers/6a6ff26c5d37378ac1dcff02 https://doi.org/10.1073/pnas.91.25.12223

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Implication

Gene expression profiling reveals distinct homeobox gene patterns in human bone marrow CD34+ subpopulations, indicating a regulated developmental program in primitive blood cells.

Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells.

Discussion

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Implication

Key Points

Cite This Study

Differential expression of homeobox genes in functionally distinct CD34+ subpopulations of human bone marrow cells.

Discussion

Member takes

Implication

Key Points

Cite This Study

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