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October 10, 2000Proceedings of the National Academy of SciencesOpen Access

Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells

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Authors

MSMaya SchuldinerWeizmann Institute of ScienceOYOfra YanukaHebrew University of JerusalemJIJoseph Itskovitz‐EldorTechnion – Israel Institute of Technology

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Implication

In vitro experimental study demonstrates distinct germ layer differentiation patterns from eight growth factors in human embryonic stem cells, suggesting strategies for targeted tissue enrichment.

Key Points

  • To assess how eight specific growth factors influence the in vitro differentiation of human embryonic stem cell-derived aggregates across different embryonic lineages.
  • Treated human embryonic stem cell-derived embryoid bodies with eight growth factors: bFGF, TGF-beta1, activin-A, BMP-4, HGF, EGF, betaNGF, and retinoic acid.
  • Assayed receptor expression and observed transitions between epithelial and mesenchymal morphologies.
  • Profiled lineage differentiation by tracking 24 molecular markers spanning ectodermal, mesodermal, and endodermal fates across 11 tissue types.
  • Activin-A and TGF-beta1 primarily induced mesodermal cell markers.
  • Retinoic acid, EGF, BMP-4, and bFGF activated both ectodermal and mesodermal lineage markers.
  • NGF and HGF supported differentiation into all three embryonic germ layers, including endoderm, though no factor drove differentiation exclusively to a single cell type.

Cite This Study

Schuldiner et al. (2000) studied this question.

synapsesocial.com/papers/6a6ff36ffebe604dd708c472https://doi.org/10.1073/pnas.97.21.11307
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  1. 1Multiple hematopoietic lineages develop from embryonic stem (ES) cells in culture1991 · 609 citations
  2. 2Functional Characteristics of ES Cell–derived Cardiac Precursor Cells Identified by Tissue-specific Expression of the Green Fluorescent Protein1998 · 185 citations
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  5. 5In vitro generation of hematopoietic stem cells from an embryonic stem cell line.1995 · 145 citations