Identification of nucleus‐encoded F₀I protein of bovine heart mitochondrial H⁺‐ATPase as a functional part of the F₀ moiety

The F0I protein of apparent Mr 27,000, previously characterized [(1988) Eur. J. Biochem. 173, 1-8] as a genuine component of bovine heart F0, has been sequenced and shown to be identical with the nucleus encoded 24,668 Da protein characterized earlier [(1987) J. Mol. Biol. 197, 89-100]. It is directly shown by proteolytic cleavage and reconstitution experiments that this protein, denoted here as PVP from the single-letter codes of the last three residues of the N-terminus, is involved in proton conduction by F0 and in its sensitivity to oligomycin.