What question did this study set out to answer?

To assess the diagnostic accuracy of a Candida albicans peptide nucleic acid fluorescent in situ hybridization (PNA FISH) probe for detecting yeast directly from positive blood culture bottles.

June 1, 2005Journal of Clinical MicrobiologyOpen Access

Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures

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To assess the diagnostic accuracy of a Candida albicans peptide nucleic acid fluorescent in situ hybridization (PNA FISH) probe for detecting yeast directly from positive blood culture bottles.
Conducted a multicenter evaluation using aliquots from 244 clinical blood culture bottles displaying yeasts on initial Gram stain.
Assayed the specimens using a standardized C. albicans PNA FISH probe assay.
The C. albicans PNA FISH test exhibited a sensitivity of 99% and a specificity of 100%.
The positive predictive value was 100% and the negative predictive value was 99.3%.

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Implication

Multicenter evaluation demonstrates high diagnostic accuracy for a Candida albicans PNA FISH probe in blood culture bottles, highlighting its utility for rapid fungal identification.

Cite This Study

Wilson et al. (2005) studied this question.

synapsesocial.com/papers/6a6ff53235aa2c282ce14337 https://doi.org/10.1128/jcm.43.6.2909-2912.2005

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Implication

Multicenter evaluation demonstrates high diagnostic accuracy for a Candida albicans PNA FISH probe in blood culture bottles, highlighting its utility for rapid fungal identification.

Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures

Key Points

Discussion

Implication

Cite This Study

Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures

Key Points

Discussion

Implication

Cite This Study

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