What question did this study set out to answer?

Characterize the epidemiology, antifungal susceptibility patterns, and virulence profiles of Cryptococcus isolates collected from AIDS patients.

September 15, 2016Mycoses

Molecular identification, antifungal resistance and virulence of Cryptococcus neoformans and Cryptococcus deneoformans isolated in Seville, Spain

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Characterize the epidemiology, antifungal susceptibility patterns, and virulence profiles of Cryptococcus isolates collected from AIDS patients.
Evaluated 28 Cryptococcus strains recovered from 12 AIDS patients over two years at a single center in Spain.
Conducted antifungal susceptibility testing via CLSI protocols and molecular typing using serotyping, mating type, PCR fingerprinting, rDNA sequencing, and ERG11 gene sequencing.
Assessed in vivo virulence variations using a Galleria mellonella larval infection model.
Cryptococcus deneoformans and C. deneoformans × C. neoformans hybrids accounted for 65% of isolates, while C. neoformans represented 35%, comprising 13 microsatellite genotypes and mixed infections in three patients.
Fluconazole resistance occurred in 29% of isolates, with one patient displaying resistance linked to an ERG11 point mutation causing a G470R substitution.
C. neoformans strains demonstrated significantly greater larval killing efficacy in G. mellonella compared to C. deneoformans and interspecies hybrid strains.

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Implication

Observational study reveals high fluconazole resistance and variable virulence among Cryptococcus strains in AIDS patients, highlighting the need for precise molecular typing.

Cite This Study

Gago et al. (2016) studied this question.

synapsesocial.com/papers/6a6ff6a184cc45bb7bdfaf90 https://doi.org/10.1111/myc.12543

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Implication

Observational study reveals high fluconazole resistance and variable virulence among Cryptococcus strains in AIDS patients, highlighting the need for precise molecular typing.

Molecular identification, antifungal resistance and virulence of Cryptococcus neoformans and Cryptococcus deneoformans isolated in Seville, Spain

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Discussion

Implication

Cite This Study

Molecular identification, antifungal resistance and virulence of Cryptococcus neoformans and Cryptococcus deneoformans isolated in Seville, Spain

Key Points

Discussion

Implication

Cite This Study

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