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Amylase polymorphism was studied by electrophoresing crude protein extracts from mature leaves of Citrus and some related genera in polyacrylamide gel slabs. A total of 25 isoenzymes distributed within 2 distinct zones were resolved. Cultivars of C. medica were the least polymorphic with 5 isoenzymes, those of C. paradisi were the most polymorphic with 14 isoenzymes; the other taxa were between these two extremes. Cultivars within species, in general, had no qualitative differences in their isoenzymes profiles with the exception of C. aurantifolia, C. limon , and C. aurantium . Certain isoenzymes showed quantitative differences among taxa. Data provided evidence substantiating the presumed hybrid origin of some taxa. The potential of isoenzyme profiles in taxonomic and genetic studies was discussed.
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Esen et al. (1977) studied this question.
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